Reproduction of Visible Absorbance Spectra of Highly Scattering Suspensions within an Integrating Sphere by Monte Carlo Simulation

It is important to avoid the overestimation of absorption due scattering when using spectroscopy measure samples. We approached this issue by placing sample inside an integrating sphere (IS) collect scattered light in all solid angles but encountered difficulty determining coefficient from absorbance because took various paths IS and sample. Therefore, ray tracing Monte Carlo simulations (MC), we estimated relationship between absorption, scattering, anisotropy coefficients, measured absorbance. Scattering M, prepared mixing polystyrene microspheres with trypan blue solution, pure solution for comparison were used as samples at concentrations. MC reproduced measurement results spectrum its concentration dependence 591 nm up limit value. In addition, saturated M was lower than that solution. This because, distribution distance d, passed through MC, more smaller d detected higher concentration, resulting a increase coefficient.